Copper Acetate Manufacturers(WSDTY) proposed all the chemical reagents used in this experiment were of analytical grade purchased from Merk chemicals. The Hibiscus rosa-sinensis flowers were collected from in and round Nehru Memorial College Puthanampatti, Tiruchirappalli, Tamil Nadu, India by using sterile polythene bages.The extract was then filtered using Whatman’s No.1 filter paper. The filtrate was collected in in a clean and dried conical flask by standard sterilized, filtration method and was stored.
For the CuO nanoparticles synthesis, 15 ml of Hibiscus rosasinensis flower extract was added to 200 ml of 1 M aqueous Copper Acetate solution in a 250 ml flask and then stirred three hours, the change in colour shows.Thus colour change indicates reduction and reduced Copper Oxide nanoparticles were obtained. The CuO nanoparticles solution thus obtained was purified by repeated centrifugation at 8,000 RPM for 15 min followed by re-dispersion of the pellet in deionized water. Then the CuO nanoparticles were dried in oven at 75°C.
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