troubleshooting of ada bridging elisa | Type 1 anti-idiotypic antibodies are neutralizing antibody in immunoglobulin format. They can be used as a control for human anti-drug antibody (ADA) in bridging ELISA. Like with any other biological assay, bridging enzyme-linked immunosorbent assay (ELISA) can be influenced by several factors, some of which can cause problems if not watched carefully. The following content will discuss the most common problems and provide you with possible solutions to solve the issue. When trouble shooting, prepare all fresh buffers, sterile filter buffers containing BSA and allow all reagents to reach room temperature prior to use. |