BSA, bulk segregant analysis, is a QTL mapping technique for identifying genomic loci affecting a trait of interest. The technique involves forming two groups crossing by individual in the extremely opposite phenotypes or a mutant of interest is crossed to wild-type, then two bulks are created by selecting individuals from the tails of the phenotypic distribution followed by pool sequencing, then the allele frequencies are estimated for the two bulks, differences would be exhibited between the two bulks if the regions of genome containing causal loci.