Poly(A) RNA sequencing (RNA-seq) is a next-generation sequencing-based method to comprehensively analyze RNAs with poly(A) tails such as message RNAs (mRNAs) and long non-coding RNAs (lncRNAs). This technique accurately reveals the level of gene expression in your biological sample under specific conditions and generates a complete picture of the transcriptome for the identification of novel alternative splicing, transcripts, gene fusion events, etc.