Boiling disinfection: use a boiling pot or a boiling sterilizer, boil for 5 to 15 minutes after boiling the water, or add 2% carbolic acid to the water for 5 minutes, add 0.02% formaldehyde, and sterilize at 80 ℃ for 60 minutes Purpose, but when selecting boiling disinfection extender, pay attention to the corrosiveness of the items. How to use the serum coagulator. If necessary, the ring and the connection between the needle and the rod should be burned. 2. After each batch of culture medium is prepared, a sterile growth test and a growth test of the tested strains should be performed.
There are buffer rooms and sliding doors corresponding to the size of the sterile room. When inoculating bacteria or samples, the pipette must be sterilized by flame after removing the pipette from the package and opening the test tube plug. (1) Take out the items, and then check the integrity of the package. (2) Make the medium into a bevel or a high layer as required.
After cryo rack in medium for 24h, it was washed by boiling. Work clothes and work cap must be worn when inoculating bacteria. Contaminated work clothes or work clothes, caps, masks, etc. When sucking the bacterial solution or sample with a straw, use the corresponding rubber tip to suck it, and do not directly suck it with your mouth. The lamp tube needs to be gently wiped with an alcohol cotton ball every two weeks to remove the dust and grease on it to reduce the impact of ultraviolet penetration.7 square meters in order to transfer items after entering the sterile room. 8. (1) When liquid samples are inoculated, they should be thoroughly mixed and sucked according to the amount for inoculation. 4.
The reaction should be normal. Should be tested again. The straws, plates and culture media used for inoculation must be sterilized and sterilized. The pH of the medium must be accurate, otherwise it will affect the growth of microorganisms or the observation of results. The inoculation loop and needle should be burned with flame before the inoculation of bacteria. ② Insert the exhaust pipe on the top cover into the square tube on the inner wall of the disinfection bucket.