Growth Curve of Copper Acetate

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asked Apr 12 in 3D Segmentation by weishida (1,240 points)

Copper Acetate Manufacturers(WSDTY) proposed for growth curves of Escherichia coli MG1655 and Copper Acetate, minimal MOPS medium, containing 1 mM MgSO4, 3 mM KH2PO4, 7.5 mM (NH4)2SO4, 30 mM NaCl, and 30 mM MOPS (pH 7) (27), was used. After autoclaving and cooling under N2, 5 mg/liter thiamine and 0.5 mM histidine were added from filter-sterilized stock, along with the vitamin and mineral mixtures described above. The medium was stored in the anoxic chamber until use. Before inoculation, 0.3% glucose was added to E. coli cultures, and fumarate and lactate (final concentrations, 20 mM each) were added for S. oneidensis. Cultures were grown in sealed Balch tubes at 37C. LB broth was used for routine aerobic growth.

For CFU counts, freshly inoculated cultures were serially diluted in anoxic YPS-MOPS medium and were plated onto YPS-MOPS plates (YPS-MOPS medium with 15% Bacto agar). Plates were grown at 30C under light in a Coy anoxic chamber for 5 days, and the resulting colonies were counted.

Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products.Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.

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