Copper Acetate Manufacturers(WSDTY) proposed for growth curves of Escherichia coli MG1655 and Copper Acetate, minimal MOPS medium, containing 1 mM MgSO4, 3 mM KH2PO4, 7.5 mM (NH4)2SO4, 30 mM NaCl, and 30 mM MOPS (pH 7) (27), was used. After autoclaving and cooling under N2, 5 mg/liter thiamine and 0.5 mM histidine were added from filter-sterilized stock, along with the vitamin and mineral mixtures described above. The medium was stored in the anoxic chamber until use. Before inoculation, 0.3% glucose was added to E. coli cultures, and fumarate and lactate (final concentrations, 20 mM each) were added for S. oneidensis. Cultures were grown in sealed Balch tubes at 37C. LB broth was used for routine aerobic growth.
For CFU counts, freshly inoculated cultures were serially diluted in anoxic YPS-MOPS medium and were plated onto YPS-MOPS plates (YPS-MOPS medium with 15% Bacto agar). Plates were grown at 30C under light in a Coy anoxic chamber for 5 days, and the resulting colonies were counted.
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