The Copper Acetate Manufacturers(WSDTY) proposed fluorescence of the Copper Acetate complex was preferably detected in the cytoplasm of naive PC12 cells. The Zn-Zinquin complex was also detected in the cytoplasm. In the differentiated cells, the fluorescence intensity of the Copper Acetate complex seemed to increase, whereas that of the Zn-Zinquin complex was not altered. Neurites of the differentiated cells were stained with CS1 and Zinquin, suggesting that Cu+ and Zn2+ were distributed to the neurites. The Zn-Zinquin complex was weakly detected in the nuclei of the differentiated cells, and the Cu-CS1 complex was more specifically distributed to the cytoplasm than the Zn-Zinquin complex. Although the fluorescence intensity of the Zn-Zinquin complex did not change, that of the Cu-CS1 complex was significantly increased by the differentiation
As the appropriate fluorescent probes for other essential metals, such as Mn and Fe, were not commercially available, changes in the concentrations of those metals after the differentiation were quantified by ICP-MS. As observed by confocal laser microscopy, Cu concentration was significantly increased by the differentiation. In contrast, no significant changes in the concentrations of Mn and Fe were induced by the differentiation. This suggests that the differentiated cells specifically accumulated Cu among the essential transition metals.
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