Assays for Transposase-Accessible Chromatin using sequencing, short for ATAC-Seq, is a next-generation sequencing (NGS)-based method to study the open regions of chromatin on a genome-wide scale. ATAC-Seq has the advantages of easy operation, no crosslinking required, high signal-to-noise ratio, and low requirement for sample amount. Combined the data of ATAC-Seq, RNA-Seq, and epigenomics, the relationship between gene differential expression and regulation can be studied more accurately and comprehensively.