In the process of transcriptomic research, the main purpose of our high-throughput sequencing is to find genes expressed differentially and explore the possible functions of these genes. For example, comparing the tissue expression profiles of diseased and normal individuals, it is reasonable to expect that the genes showing significant differences are related to the occurrence of diseases. These genes may be involved in immune-related biological processes, signal pathways, and gene expression levels. Therefore, after identifying these genes, the pathways involved are often annotated. Through GO or KEGG enrichment analysis, these differentially expressed genes can be mapped to GO or KEGG classification entries, so as to understand the regulatory pathways involved in these genes, and finally correlate with the phenotype.