UMI small RNA sequencing (RNA-seq) is a unique molecular identifier (UMI)-based technology for accurate qualitative and quantitative analysis of multiple small RNAs in cells. PCR amplification bias can be removed by adding UMI into each cDNA segment, achieving accurate and unbiased quantification. UMI small RNA-seq can accurately identify SNP, quantify low-abundance transcripts, and reveal comprehensive transcriptome information. We provide UMI small RNA-seq to help you analyze microRNA (miRNA), small interfering RNA (siRNA), piwi-interacting RNA (piRNA) at one time.