Oligonucleotides are polymer sequences of nucleotides (RNA, DNA and their analogues). Health diagnosis and drug gene research were carried out by using primers and siRNA, oligonucleotides for basic molecular research, as well as microarray and fluorophore probes. Fluorophore is a very useful biomolecule sensitive marker, and fluorescent labeling is the most commonly used synthetic oligonucleotide marker so far. Many different fluorescent groups can be easily connected to oligonucleotides, which form the basis of the detection systems used in DNA sequencing, forensic medicine, and genetic analysis.