Fluorophore is fluorescent chemical compounds that are highly conjugated and can re-emit fluorescence upon light excitation. Fluorophore can be used alone as a tracer, a probe or a substrate for enzyme. While covalently bonding with macromolecules, it serves as a marker and help quantification and tracking of the biomolecules by fluorescent imaging. The non-constructive nature and high sensitivity of fluorescence technique makes fluorescent labeling one of the most advantageous method to track biomolecules in many ways.