With the more and more extensive and in-depth application of peptides in the field of biomedicine, the demand for labeled and modified peptides is more and more, and the quality demand is higher and higher. Stable isotope marker is a typical one. Stable isotope labeling can realize the study of the metabolic pathway of peptides, and can track the changes of the position and quantity of polypeptides containing isotope markers in vivo or in vitro at any time. Isotope labeling has the advantages of high sensitivity, simple location and accurate quantification, so isotope modification has been paid more and more attention in the fields of medicine and biochemistry.