BCL6 Break Apart Probe
Product Advantages
1. Fleetness: Tissue probe hybridization time: 2 hours. Cell probe hybridization time: 1 hour.
2. Accuracy: Less non-specific background staining (dyeing). Increase difficult samples detection rate.
3. Reproducibility: Different laboratories test results are highly reproducible.
◆Probe Description
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BCL6 Break Apart Probe is a dual-color break probe that is a mixture of two probes directly labeled to 3q27.3-q28. The green fluorescent directly labeled probe hybridizes with the 3q27.3 proximal BCL6 gene, while the orange is red. The fluorescently labeled probe directly hybridizes to the distal end of the BCL6 gene of the 3q27.3-q28 distal group.
Translocation of the BCL6 gene occurs in 6-26% of follicular lymphoma (FL)[1] with higher incidence (44%) in grade 3 cases negative for t(14;18)(q32;q21). Rearrangement of the BCL6 gene is observed at a frequency of 15~40% in diffuse large B-cell lymphomas (DLBCL).
This probe is used to detect whether the BCL6 gene is broken and translocated. BCL6 gene fragmentation is an independent indicator for evaluating survival rate and recovery rate.
◆Clinical Significance of BCL6 Break Apart Probe
In diffuse large B-cell lymphoma, BCL6 gene can translocate with multiple genes, the incidence rate is 20%-40%; in follicular lymphoma, the incidence rate is 5-15%. Burkitt's lymphoma is morphologically suggestive of typical age, morphology, and immune characterization. If any of these three characteristics are not typical or have a history of follicular lymphoma with MYC gene breaks and BCL6 gene breaks, Gray area lymphoma between Burkitt/DLBCL should be diagnosed.
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