Treatment method: add an appropriate amount of DTT or Beta mercaptoethanol after heating and boiling to supplement the insufficient reducing agent; add an appropriate amount of EDTA to prevent the oxidation of the reducing agent. Solution: Add an appropriate amount of buffer solution between the two plates to eliminate air bubbles.
The target zone is large and sometimes cannot enter the separation gel. However, it has the same immunological activity as the target zone, and it can be seen in the immunoblot reaction that it can act as an antibody corresponding to the target zone. 8. Solution: Replace the buffer with the correct pH value to reduce the concentration of the separation gel.
The electrophoretic voltage is very high and the current is very low: The reason for the formation: mainly because the electrophoresis tank is not properly assembled and the current does not form a path. Handling method: Centrifuge before adding the sample, and add an appropriate amount of sample promoting solvent. Solution: The electrophoresis tank is correctly assembled. Ghost belt: Cause of formation:
Mainly because the reducing agent is oxidized during heating and loses its activity, which causes the original dissociated protein molecules to refold and combine and subunits to re-associate to polymerize into large molecules. Third, the zone tailing: Formation reasons: mainly caused by poor sample dissolution or excessive gel concentration.8), and reduce the voltage appropriately.. Six, bromophenol blue can not give an indication: Reason for formation: In the experiment, the phenomenon that bromophenol blue has run out of the bottom of the plate, but the protein has not run down is mainly cryotube related to the concentration of the buffer solution and the separation gel.I. V. Fourth, the texture of the zone: Formation reasons: mainly caused by sample insoluble particles. Treatment method: wait for it to fully solidify before experimenting. The zone is very thick: Reason for formation: Mainly because the concentrated gum is not concentrated well