So far, the company has a human-sourced spot plasmid library (18 000), an adenovirus spot library (12 000), and an adeno-associated virus (AAV) spot library. 6, hands or relatively dirty items can not pass through the open bottle mouth, that is, can not be operated above the open container. 4. The process of recovery should melt quickly, the purpose is to prevent small ice crystals from forming large ice crystals, that is, recrystallization of ice crystals. Open the cryopreservation tube and aspirate the cell suspension into a centrifuge tube. Sincerely welcome your consultation and purchase!. The company also has a wealth of customized services.
Cells from the proliferative phase to the formation of dense monolayers can be used for cryopreservation, but preferably in the logarithmic phase. 2. Knockout, gene mutation, etc. When pipetting cells, pay attention to whether the cells in the corners are pipetting down. 2. Pipette the cell suspension after passage, centrifuge, remove the culture solution, add the cryopreservation solution, and aliquot the cryopreservation tube (the number of cells in the cryopreservation tube is generally (5 ～ 10) times; 106 Cells / ml, 1 ~ 1. This can ensure that the extracellular crystals melt in a short period of time, avoiding the infiltration of water into the cells due to slow melting to form intracellular recrystallization and damage to cells. Special care should be taken for cell lines derived from human or viral infections.
Cryopreservation method 2: The freezing tube is placed in a program cooling machine with a set program, and the temperature is lowered by 1 to 3 ° C to -80 ° C per minute, and then stored in liquid nitrogen for a long time. Pay attention to your own safety. Cryopreservation of cells 1. 4. When the cells are cooled below zero, the following changes lab disposable products can occur: the organelles are dehydrated, the concentration of soluble substances in the cells increases, and ice crystals are formed in the cells. The service items include: scientific and clinical grade adenovirus, lentivirus, adeno-associated virus (AAV) packaging, plasmid vector construction,
TALEN gene.5ml cells are usually placed in 2ml cryopreservation tubes). If it has contacted, use flame burning to disinfect or replace the spare parts. It is best to change the culture medium one day before freezing. 2. When putting the cryopreservation tube into or removing from the liquid nitrogen container, protect it to prevent frostbite. When opening and closing the culture flask with cells, the flame sterilization time should be short.25% trypsin, ultra clean bench, carbon dioxide incubator, inverted microscope, microscope, counting plate, centrifugation Machine